- Process Equipment
- Microbiological Anaerobic Chamber
- Hydrogen Storage Equipment
- Gas cylinder
Several procedural and design changes should be considered for the future:
Replace the use of pure hydrogen with a 95:5 mixture of nitrogen and hydrogen to reduce the possibility of an explosive atmosphere occurring. Laboratory personnel should check each tank that is delivered to ensure that the gases are present in the proper ratio.
Adhere to the manufacturer's recommendations for operation of the anaerobic chamber.
Following the check of the lines to make sure all the connections are tight, all gas cylinders should be closed; then, only the desired gas cylinder should be opened for use.
Use of "T" connections between gases should be eliminated. If there is continued use of a "T" connection, only connections with a toggle switch to limit the introduction of gas from a single cylinder should be used. No exceptions, even on a temporary basis.
The laboratory should continue to investigate the availability of hydrogen and/or oxygen sensors with the hope of finding some that can withstand the corrosive atmospheric environment.
All laboratory personnel should receive refresher training that includes standard safety precautions as well as a more detailed review of the hazards of working with hydrogen. Hydrogen use in anaerobic chambers is discussed in the Lessons Learned Corner on this website.