This study is aimed at the validation of the pressure peaking phenomenon against laboratory-scale experiments. The phenomenon was discovered recently as a result of analytical and numerical studies performed at Ulster University. The phenomenon is characterized by the existence of a peak on the overpressure transient in an enclosure with vent(s) at some conditions. The peak overpressure can significantly exceed the steady-state pressure and jeopardise a civil structure integrity causing serious life safety and property protection problems. However, the experimental validation of the phenomenon was absent until recently. The validation experiments were performed at Karlsruhe Institute of Technology within the framework of the HyIndoor project (www.hyindoor.eu). Tests were carried out with release of three different gases (air, helium, and hydrogen) within a laboratory-scale enclosure of about 1 m 3 volume with a vent of comparatively small size. The model of pressure peaking phenomenon reproduced closely the experimental pressure dynamics within the enclosure for all three used gases. The prediction of pressure peaking phenomenon consists of two steps which are explained in detail. Examples of calculation for typical hydrogen applications are presented.